Cytokinin and Metabolites Affect Rhizome Growth and Development in Kentucky Bluegrass (Poa pratensis)

Rhizome growth and development is regulated by phytohormone. However, endogenous phytohormones affect rhizome initiation, sustained in perennial grass species remains elusive. In this study, we investigated the morphological characteristics content of indole-3-acetic acid (IAA), zeatin (ZT), gibberellic (GA3), abscisic (ABA) rhizomes two different Kentucky bluegrass. Using ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS), performed metabolite analysis rhizomes. our multi-rhizome bluegrass material ‘Yuzhong’ had an average 1113 rhizomes, while few-rhizome ‘Anding’ 347 The diameter length internode were 1.68-fold 1.33-fold higher than that ‘Anding’, respectively. dry weight was 75.06 g, 20.79 g. a ZT (5.50 μg·g−1), which 2.4-fold (2.27 μg·g−1). contrast, IAA, ABA, GA3 markedly ‘Yuzhong’. Correlation revealed significant correlations between ZT/ABA with number rhizome, internode, whereas GA3, IAA/ZT opposite. metabolic profiles, identified 163 differentially expressed metabolites (DEMs) (60 upregulated 103 downregulated) positive ion mode 75 DEMs (36 39 negative mode. Histidine metabolism ABC transporters pathways most significantly enriched mode, respectively, both are involved synthesis transport cytokinin. These results indicate cytokinin crucial for promotes germination